June 4 - 6, 2012
Crowne Plaza Hotel
St. Louis, MO
Abstracts
Veterinary Drug Residues in Tissues,
Session Co-Chairs: Dr. Sheri Turnipseed and Terry Dutko

  • Alan R. Lightfield, USDA Agricultural Research Service; Wyndmoor, PA
    Lucía Geis-Asteggiante, USDA Agricultural Research Service; Wyndmoor, PA and Cátedra de Farmacognosia y Productos Naturales, DQO; Facultad de Química;
    UdelaR; General Flores 2124; 12800, Montevideo; Uruguay
    Steven J. Lehotay, USDA Agricultural Research Service; Wyndmoor, PA

    Optimization of an UHPLC-MS/MS identification-based screening method for >120 veterinary drug residues in target tissues


    Recently, a practical and reliable multiclass, multiresidue method was developed by our research group for the qualitative identification of 62 priority veterinary drug
    residues by UHPLC-MS/MS in bovine kidney.  This method has been implemented for regulatory purposes by the USDA Food Safety Inspection Service for lab-based
    screening of presumptive positive samples from slaughter establishments.  Meanwhile, we’ve optimized and tested the method to screen for an additional 66 drug
    analytes, which was validated in beef and other target tissues.  The optimization study centered on extensive ruggedness evaluation by evaluating different clean-up
    sorbents, matrix effects, and dilution factors vs. detection limits.  Although the method is designed for identification screening only, quantitative recoveries and
    reproducibility were also determined.  A multi-day, multi-analyst validation was done using the final method, which obtained good qualitative performance and
    demonstrated that the method is suitable for screening purposes at or below US regulatory tolerance levels.
    _________________________________________________________________________________________________________________________________________
    Alan R. Lightfield (1), Lucía Geis-Asteggiante(1,2), and Steven J. Lehotay (1)
    (1) USDA Agricultural Research Service; 600 East Mermaid Lane; Wyndmoor, PA  19038
    (2) Cátedra de Farmacognosia y Productos Naturales, DQO; Facultad de Química; UdelaR; General Flores 2124; 12800, Montevideo; Uruguay